00:21:06 Jacquelyn Stenman: Jacquelyn Stenman, Jefferson County Public Health 00:21:07 Jenny Wolfe: Jenny Wolfe (she/her), Dept of Ecology. Burfoot Park Olympia 00:21:07 rick fuller: Hiya, direct from southern arizona 00:21:08 Henry Hua: Henry, Northwest Indian Fisheries Commission, Salmon Biologist, I love Lake Crescent! 00:21:20 Michael Dawson: Mike Dawson, Jefferson County, Fort Worden State Park 00:21:25 Andy Suhrbier: Andy Suhrbier, Pacific Shellfish Institute, Alpine lakes 00:21:41 Amanda Hendrix (she/her) WSDA: Amanda Hendrix, WSDA, Lake Whatcom 00:21:42 Leah Shamlian: Leah Shamlian, WA Dept of Ecology 00:21:45 Tanya Truong: Tanya Truong, Tacoma-Pierce County Health Department 00:21:51 Leanne Leanne: Leanne Ingman, Skagit County, Baker Lake 00:21:55 Roger Parker: Roger Parker, WA DOH, Fort Worden 00:22:06 Niamh O'Rourke: Niamh O'Rourke, WA Dept of Ecology, Seahurst Beach 00:22:06 Shannon Valderas: Shannon Valderas, Friends of the Salish Sea. Lake Chelan 00:22:08 Natalie Prystajecky: Natalie Prystajecky BCCDC Public Health Lab, Shuswap Lake 00:22:12 Amanda Royal: Amanda Royal, City of Bothell, Moran State Park 00:22:13 Chelsea Morris (she/her), Ecology: Chelsea Morris, Ecology, any alpine lake 00:22:14 Kirsten McDade: Kirsten McDade, North Sound Waterkeeper, RE Sources. Alpine lakes. 00:22:15 Kaylin Gentz: Kaylin Gentz, Dept of Ecology, Baker Lake 00:22:16 Andrea Watts: Andrea Watts, The Daily World 00:22:25 Ian Tracy: Mason County Public Health. I prefer to swim in my pool haha 00:22:28 Daniel Nidzgorski: Daniel Nidzgorski, King County Natural Resources, and I miss the warmer ocean I grew up with in NJ 00:22:35 Jamie Wasielewski: Jamie Wasielewski, Ecology, Olympia 00:22:35 Matthew Colston: Matthew Colston, WA Dept of Ecology 00:22:36 Heather McFarlane: Heather McFarlane, Friends of BurleyLagoon, Pierce County 00:22:37 Trevor Swanson: Trevor Swanson, DOH Shellfish, Park Lake, E WA 00:22:38 Diane Yeh: Diane, King County Water Land Resources Division 00:22:44 Zoe Fry: Zoe Fry, Program Coordinator at RE Sources, Bellingham, Whatcom Creek 00:22:46 Jill Stewart: Jill Stewart, WA DOH 00:22:58 Chris Woos: Chris Wood here. I manage data and violation notifications to stormwater managers for the Salish Sea Stormwater Monitoring Program. A volunteer based monitoring program in 7 Phase II Permit cities along the shoreline of Salish Sea 00:23:05 Ingrid Enschede: Ingrid Enschede, Whatcom County Public Works 00:23:06 Aubrey Cardoza: Aubrey Cardoza, Mason Conservation District 00:23:07 Christy Juteau: Christy Juteau, KWL Biologist working with Semiahmoo First Nation and Shared Waters Alliance to revitalize shellfish harvest. Favourite swimming place is Crescent Beach, South Surrey BC 00:23:15 Aida Miller: Aida Miller, Skagit County Natural Resources 00:23:16 Fiona Dahl: Fiona Dahl, WA DOH Shellfish, I love swimming the Hood Canal 00:23:23 Barbara Ann Smolko: Barbara Ann Smolko, Pierce County Surface Water Management 00:23:23 Dana Archer: Dana Archer, Environmental Analyst with Port of Tacoma. 00:23:24 Don Robinett: Don Robinett, Snohomish County SWM - Lower Stillaguamish PIC 00:23:35 Tristan Olson: Tristan Olson, Nisqually River Council 00:23:39 James Kardouni: James Kardouni, Dept. of Ecology. Alpine lakes on a sunny warm day. 00:23:39 Anna Bachmann: Anna Bachmann, Puget Soundkeeper Alliance 00:23:40 Carlie Miller, Island County Public Health: Carlie Miller, Island County, Surface Water Quality 00:23:47 Kirsti L. (she,her): Kirsti Lipphardt, City of Tacoma Environmental Services 00:23:54 Austin Lymburner: Austin Lymburner, City of Abbotsford Environmental Coordinator, Cultus Lake BC 00:23:57 Richard Strickland: Richard Strickland, Friends of Salish Sea, Lake Washington 00:24:02 Tim Singh: Tim Singh, Metro Vancouver (Canada) 00:24:07 Rachel Bowen: Rachel Bowen, City of Poulsbo 00:24:22 Michael Marrs: Michael Marrs, Mason County Environmental Health Clean Water District. 00:24:24 Kim Lagimodiere: Kim Lagimodiere, Cowichan Tribes, Quw'utsun 00:24:27 Maggie Taylor: Maggie Taylor, Nooksack Indian Tribe, Natural Resources Dept 00:24:40 Rick Beauregard: Good morning. Rick Beauregard, Whatcom MRC, NW Straits Commission, Drayton Harbor Shellfish Protrection District Adv Comm. 00:24:43 Jean Frost: Jean Frost, Thurston County Environmental Health 00:24:50 Michael Isensee: Michael Isensee, WSDA Nutrient Mgmt. Technical Services. Lake of the Woods, OR. 00:24:50 Mark Whelly: Mark Whelly, Hatfield Consultants working with Semiahmoo First Nation, BC 00:24:52 Rebecca Mahan: Rebecca Mahan, Clallam County 00:24:57 Lea Shields: Good morning all! Lea Shields, WA Dept of Health National Estuary Program Shellfish Strategic Initiative 00:24:58 Zachariah Van Ry: Zach Van Ry, King County Stormwater. 00:25:08 Christian Berg: Christian Berg, City of Bainbridge Island 00:25:30 Lindsay Tuttle: Lindsay Tuttle, TPCHD 00:25:34 Liora Llewellyn: Morning! Liora Llewellyn, King County Stormwater 00:25:47 Wenfei Ni: Wenfei Ni, Pacific Northwest National Lab/University of Washington-Tacoma 00:26:19 Christopher Krembs: Christopher Krembs Washington State Dept of Ecology, Marine Monitoring 00:27:42 Bill Lawrence: Good morning! Bill Lawrence, King County Environmental Lab, Microbiology 00:28:53 Wendy Eash-Loucks: Wendy Eash-Loucks, King County, Marine and Sediment Assessment Group 00:29:20 Silvia Rus: Good morning! Silvia Rus, King County, On-site Sewage System Program 00:29:32 Marielle Kanojia: Marielle Kanojia (she/her) UW Puget Sound Institute. My favorite place to swim in the summer is down near Shelton 00:29:43 Lizbeth Seebacher: Lizbeth Seebacher, University of Washington 00:31:00 Raye Evrard: Raye Evrard (she/her) UW Puget Sound Institute. My favorite place to swim is Magnusen Park in Seattle! 00:32:00 Lynn Schneider: Lynn Schneider - King County OSS Permitting Program 00:32:54 Breanna McArdle: Breanna (Bre) McArdle - WA DOH, epidemiologist and program manager for the Washington Wastewater-Based Epidemiology Program. 00:33:02 Katherine Garrah: Katherine Garrah (Kat), Drayton Harbor Oyster Company in Blaine - I love to swim in Semiahmoo Bay 00:33:04 Amy Georgeson: Amy Georgeson (she/her) City of Battle Ground, Stormwater Program Coordinator, the Washougal River is my current favorite swim spot. 00:34:02 Theo Jones: Good morning! Theo Jones, WA Dept of Health National Estuary Program Shellfish Strategic Initiative, Administrative Assistant 00:34:04 Brad Bebout: Brad Bebout, Port Townsend. Interested citizen. 00:34:37 Amanda Gawor: Good morning, Amanda Gawor, Hatfield Consultants, North Vancouver Canada 00:35:04 Bobbi Hudson: Bobbi Hudson, Exec Dir of Pacific Shellfish Institute, based in Olympia WA 00:36:24 Keya Sen: Keya Sen, Faculty , IAS, UW Bothell (Retired Senior Microbiologist, US EPA , Office of Water). 00:38:00 James Packman, Herrera Environmental Consultants: James Packman, hydrologist and water quality scientist, Herrera Environmental Consultants. Favorite swim spot: coves on Manitoulin Island, Ontario (Lake Huron) 00:39:16 Alex Johnson: Alex Johnson, Chief Strategy Officer, Virridy. Also Fellow at the Mortenson Center in Global Engineering & Resilience, Fellow at Forest Trends, and Commissioner at the Columbia River Gorge Commission. Favorite place to swim is the Deschutes River in OR. 00:39:56 Alan Chapman: Alan Chapman WRIA 1 Planning Unit, Fisher Caucus 00:48:11 Kim Lagimodiere: Unfortunately I must step away. Is this meeting being recorded or will the slides be shared with participants? 00:48:23 Marielle Kanojia: Replying to "Unfortunately I must..." Yes, we are recording and will also share the slides 00:48:26 Zachariah Van Ry: Replying to "Unfortunately I must..." Yes it is. 00:48:30 Kim Lagimodiere: Replying to "Unfortunately I must..." Thank you! 00:49:09 Anna Bachmann: yeah, waterkeepers! 00:49:25 Michael Dawson: Reacted to "yeah, waterkeepers!" with πŸ‘πŸ» 00:49:54 Anu Rao: Reacted to "Yes, we are recordin..." with πŸ‘πŸ½ 00:50:06 Amanda Gawor: Reacted to "Yes, we are recordin..." with πŸ‘ 01:02:07 George Onyullo: Reacted to "Yes, we are recordin..." with πŸ‘πŸ½ 01:02:28 Zachariah Van Ry: Click use slide show 01:02:36 Theresa Mitchell: click the "swap displays" button 01:02:50 rick fuller: Replying to "Unfortunately I must..." Has anyone else used optical brightners as a source tracing method for human sewage sources? 01:02:50 Jessica Huybregts: Reacted to "click the "swap disp..." with πŸ‘ 01:03:00 Wenfei Ni: I think you can swap displays on the left top corner when you hit presenting 01:03:03 Kirsten McDade: Reacted to "yeah, waterkeepers!" with πŸ‘πŸ» 01:03:33 Nick Ng: sorry missed the early introduction Nick Ng, Natural Resource Technician, Sumas First Nation of British Columbia, Canada 01:03:40 Jenn Smith: Looks great here! 01:04:18 Cameron Chapman: Has anyone else used optical brightners as a source tracing method for human sewage sources? Limited attempts but not successful. False positives are a concern. I'm also interested to know if anyone has had any success. 01:04:45 Amanda Gawor: Replying to "sorry missed the ear..." Hi Nick! (Amanda here previously GNR Natural Resource Officer) 01:04:46 Karen DuBose: Replying to "Unfortunately I must..." Yes, without success. Our watershed is very rural, and lots of laundry detergents (the "free & clear" ones) don't have optical brighteners in them. It's a good tool in the toolkit and can be cheap to use, but it's not bullet proof. 01:05:04 Nick Ng: Reacted to "Hi Nick! (Amanda h..." with ❀️ 01:05:52 rick fuller: City of Tacoma has a lot of data showing success for the quick response OB methods. They have research papers showing methods and results. 01:06:32 Raye Evrard: Reacted to "City of Tacoma has a..." with πŸ‘πŸ» 01:07:59 James Packman, Herrera Environmental Consultants: Reacted to "City of Tacoma has..." with πŸ‘ 01:10:28 rick fuller: Replying to "Unfortunately I must..." Ran across the "clear" detergents issue. Used caffeine as secondary verification. 01:16:23 Karen DuBose: Reacted to "Ran across the "clea..." with πŸ‘ 01:16:59 Barbara Ann Smolko: If humans are eating cows, pigs, and/or chickens, could that influence the signal? 01:20:48 Natalie Prystajecky: Thanks for the great presentations. A question for later, if there's time: How often do your studies find mismatches between fecal coliforms and MST results and if you do, how do you address them? 01:22:37 Raye Evrard: Reacted to "If humans are eating..." with πŸ‘πŸ» 01:22:40 Raye Evrard: Reacted to "Thanks for the great..." with πŸ‘πŸ» 01:22:48 George Onyullo: Reacted to "Thanks for the great..." with πŸ‘πŸ» 01:22:53 George Onyullo: Reacted to "If humans are eating..." with πŸ‘πŸ» 01:22:58 George Onyullo: Reacted to "City of Tacoma has a..." with πŸ‘πŸ» 01:23:30 Keya Sen: What volume of water do your studies typically test. 01:23:56 Raye Evrard: Reacted to "What volume of water..." with πŸ‘πŸ» 01:25:25 Keya Sen: If you use larger volumes do you then reduce it per 100ml for reporting purposes? 01:26:55 Raye Evrard: Reacted to "If you use larger vo..." with πŸ‘πŸ» 01:33:49 Ingrid Enschede: Whatcom County Public Works partnered with EPA Region 10 on a MST study that included reference samples taken from septic tanks. We can share how we got those samples and more. Ingrid Enschede and Cynthia May 01:34:09 Marielle Kanojia: Reacted to "Whatcom County Publi..." with ❀️ 01:34:16 Alan Chapman: What exactly is the biofilm. Is it on the pipe surfaces, sewage surface or throughout the sample.? 01:34:30 Amy Georgeson: Reacted to "Whatcom County Publi..." with πŸ‘ 01:34:57 Raye Evrard: Reacted to "Whatcom County Publi..." with ❀️ 01:35:36 John Griffith: Biofilm is on the wetted surfaces of the pipes 01:36:09 John Griffith: We standardize to 100ml - also when we use smaller volumes 01:38:09 Whitney Knopp: https://virridy.com/lume-analytics/ 01:38:17 Nick Ng: Reacted to "https://virridy.co..." with ❀️ 01:38:27 Nick Ng: Reacted to "We standardize to ..." with πŸ‘ 01:39:20 John Griffith: Composite samples are typically taken with and autosampler. It varies, but we typically take a 250 ml sample every 10 minutes during the storm 01:40:20 rick fuller: Any data showing interferences like detergents? 01:40:52 John Griffith: What animals humans eat does not influence the human marker signal 01:43:52 John Griffith: A small amount of detergent probably would not interfere with PCR, as the water goes through the filter leaving just the bacteria behind. If that were a concern, one could always do a rinse step with PBS to remove any remaining detergent residue 01:43:53 Nick Ng: How does Lume account for false positives from organic matter that isn’t fecal in origin? 01:44:55 Daniel Nidzgorski (he/him), King County: Replying to "What animals humans ..." Some of my colleagues have reported the opposite sort of cross-contamination -- human marker showing up in raccoon poop. Raccoons living in the sewer and exposed to human poop got the HF183 strain living in their own gut microbiome. It's a good reminder that we're measuring a strain of gut bacteria that happen to live primarily in human guts, not measuring human DNA. 01:45:03 rick fuller: Reacted to "How does Lume accoun..." with πŸ‘ 01:45:54 Whitney Knopp: https://www.sciencedirect.com/science/article/pii/S0043135422005978 01:46:10 Natalie Prystajecky: Reacted to "https://www.scienced..." with πŸ‘ 01:46:17 Nick Ng: Reacted to "https://www.scienc..." with πŸ‘ 01:46:30 Michael Isensee: Any familiarity with a former commercial model ZAPs by ZAPs technology. The company went out of business in 2020, but EPA Region 10 spent a substantial sum on a pilot in Whatcom County and several had been deployed and used in Tillamook. Steven Potokar would be the contact: Steven Potokar (potokar.steven@epa.gov) Phone: (206-553-6354) 01:47:02 Don Robinett: Do we have any idea what the per sensor unit price would once it goes to market? 01:47:39 Michael Isensee: Replying to "Any familiarity with..." Their technology appeared to have big challenges in a glacial waterway with its associated turbidity. 01:48:07 Cameron Chapman: Dogs eat human poop....we have also seen dog marker show up in human poop (a human dog owner). 01:48:42 Michael Isensee: Replying to "Any familiarity with..." Here is the final report from Whatcom County, WA: https://www.whatcomcd.org/_files/ugd/5bf978_9ce453d19b8449798328f7678e5d3384.pdf 01:50:03 Alan Chapman: Does sediment effect the fluorescence? 01:50:20 Keya Sen: Is 100 ml true representative of 250 ml or more of the water sample? 01:50:22 Alex Johnson: Thanks @Don Robinett - we’re targeting a fee-for-service model rather than selling devices, ideally $250/month/unit. We’re in a lot of discussions with partners on what works best though so don’t let that be a limiting factor if you see a good use. 01:51:36 steve britsch: Question for Alex and Whitney. Interesting technology. The x and y axis in your Lume Performance correlation showed colony counts ranging from 1 - 4. Is the 20% agreement really at counts this low? Asking because it's often very difficult to achieve this level of precision at low counts whether this be for field or lab duplicates using SM9222D for E.coli. 01:52:01 Michael Isensee: Replying to "What animals humans ..." We also have seen source ID challenges where cross contamination is occurring without passing through a digestive tract. Dogs and ducks swimming in manure lagoons, then in freshwater. Loads of bird droppings entering manure storage systems at times in barns where 20,000 starlings arrive each day. 01:52:01 rick fuller: Reacted to "Question for Alex an..." with πŸ‘ 01:52:42 Andy Suhrbier: We've tried the Proteus brand coliform sensor (via tryptophan), with some success after creating an algorithm for "our" saltwater. We still need to conduct more testing as it certainly isn't perfect as the marine environment is more difficult. Would be interested in trying the Virridy unit if possible. 01:54:21 Don Robinett: Replying to "Thanks @Don Robinett..." thanks 01:55:54 John Griffith: Replying to "What animals humans ..." That sounds very challenging 01:56:08 Alex Johnson: Reacted to "We've tried the Prot..." with πŸ‘ 01:57:28 Whitney Knopp: I have to hop to another meeting, please feel free to reach out with any other questions! Thanks so much! 01:57:36 Nick Ng: Reacted to "I have to hop to a..." with ❀️ 01:57:37 Raye Evrard: Reacted to "I have to hop to ano..." with ❀️ 01:57:40 steve britsch: Reacted to "I have to hop to ano..." with πŸ‘ 01:58:29 Raye Evrard: Reacted to "Here is the final re..." with πŸ‘πŸ» 01:59:23 Michael Isensee: Reacted to "That sounds very cha..." with πŸ‘ 02:00:57 Cameron Chapman: Depending on dilution, you might get low fecal indicator bacteria but still a low or low-moderate hit on the human result. The human numeric results don't necessarily say anything about the quantity of waste present. 02:01:24 Amy Zimmer-Faust: Reacted to "Depending on dilutio..." with πŸ‘ 02:01:41 Nick Ng: Reacted to "Depending on dilut..." with πŸ‘ 02:04:11 Natalie Prystajecky: Thank you! 02:06:48 Keya Sen: Question is should your report per 250 ml or 100 ml? 02:08:53 Natalie Prystajecky: It must be fun to see the gamma radiated filter in the microscope, thanks for that breakdown! 02:09:28 Marielle Kanojia: Reacted to "It must be fun to se..." with ❀️ 02:18:52 John Griffith: Replying to "Question is should y..." By convention, per 100ml 02:19:06 Paul Vance: Any advice or tips on mixing of a larger sample to extract succcessive aliquotes to reduce inconsistency of test results on extracted samples? 02:22:09 Amy Zimmer-Faust: Replying to "Any advice or tips o..." Make sure the sample is well mixed between filters. Also, you can include spike-in controls to help identify if inconsistency/issues happen with a specific filter. 02:36:34 Amanda Royal: Can anyone expand on what GCN you would considered elevated to trigger an investigation? 02:41:34 John Griffith: Replying to "Any advice or tips o..." Unfortunately, the extortion step can often introduce variability because it depends heavily on the pipetting talent of the technician. Using an extraction robot helps, but we still see some variability between replicate extractions of the same sample 02:45:35 Alan Chapman: Are any of the initial presenters familiar with the genomic study done in Whatcom County? 02:45:50 Raye Evrard: Reacted to "Can anyone expand on..." with πŸ‘πŸ» 02:45:52 Raye Evrard: Reacted to "Are any of the initi..." with πŸ‘πŸ» 02:52:55 Raye Evrard: Please add any questions you have for the presenters to the chat or feel free to raise your hands! Thank you all for participating so far πŸ™‚. 02:54:21 Amy Zimmer-Faust: https://iopscience.iop.org/article/10.1088/2515-7620/ac693a/meta *Good study using crAssphage 02:54:30 Raye Evrard: Reacted to "https://iopscience.i..." with πŸ‘πŸ» 02:55:38 Marielle Kanojia: Andy also shared this paper - https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2023.1256174/full 02:55:41 Natalie Prystajecky: Reacted to "Andy also shared thi..." with πŸ‘ 02:55:49 Mark Whelly: Reacted to "https://iopscience.i..." with πŸ‘ 02:55:54 Natalie Prystajecky: Reacted to "Andy also shared thi..." with πŸ‘ 02:55:56 Raye Evrard: Reacted to "Andy also shared thi..." with πŸ‘ 03:04:57 Michael Dawson: EPA Manchester lab is a great resource for the Puget Sound region 03:06:40 Sarah Yepez: Reacted to "EPA Manchester lab i..." with πŸ‘ 03:09:14 Cynthia May: EPA Region 10 Manchester lab contact: Stephanie Bailey: bailey.stephanie@epa.gov 03:09:45 Trevor Swanson: Stephanie is great to work with. 03:09:53 Karen DuBose: Reacted to "Stephanie is great t..." with πŸ‘ 03:10:10 Zachariah Van Ry: Thank you everyone! 03:10:11 Raye Evrard: Thank you all for joining us today. 03:13:01 Philip Murphy: Marielle, PSI and all the presenters and question askers, thanks for this webinar - really informative! 03:13:09 Andrew James: If there were interest, we could reconvene this group, maybe annually, to try to provide information on the latest developments in MST technologies. 03:13:20 Cameron Chapman: Reacted to "If there were inte..." with πŸ‘ 03:13:23 Michael Dawson: Reacted to "If there were intere..." with πŸ‘πŸ» 03:13:32 Philip Murphy: Reacted to "If there were intere..." with πŸ‘ 03:13:43 Raye Evrard: Reacted to "Marielle, PSI and al..." with ❀️ 03:13:53 steve britsch: Reacted to "If there were intere..." with πŸ‘πŸ» 03:13:54 Raye Evrard: Reacted to "If there were intere..." with πŸ‘ 03:14:07 Bill Lawrence: Reacted to "If there were intere..." with πŸ‘ 03:14:18 Amy Zimmer-Faust: https://www.epa.gov/awma/national-water-quality-monitoring-council 03:14:40 Cynthia May: Reacted to "If there were intere..." with πŸ‘ 03:15:04 Natalie Prystajecky: Reacted to "https://www.epa.gov/..." with πŸ‘ 03:16:02 Noah Roland: Reacted to "If there were intere..." with πŸ‘ 03:17:08 Amy Georgeson: Thanks for putting together these informative presentations. Great to know about some of the advances that have been in made in MST in the last 20 or so years. 03:17:16 Raye Evrard: Reacted to "Thanks for putting t..." with ❀️ 03:17:17 Anna Bachmann: ❀️ 03:17:17 Jenn Smith: Thank you very much!